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Texas A&M cotton study identifies fiber length, strength traits

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By: Kay Ledbetter

Ongoing effort to utilize genotyping in cotton breeding advancements

Writer: Kay Ledbetter, 806-677-5608, skledbetter@ag.tamu.edu
Contact: Dr. Wayne Smith, 979-845-3450, cwsmith@tamu.edu

COLLEGE STATION – Texas A&M University soil and crop sciences department and Texas A&M AgriLife Research are looking at using molecular tools as a solution to the world cotton market’s demand for longer, stronger fibers for spinning and weaving.

Dr. Wayne Smith, associate soil and crops sciences department head in College Station, and his graduate student Kari Hugie, said their ongoing project may not be seeing big dividends right now, but could be pavingthe way for the future.

Smith said upland cotton accounts for the majority of cotton fiber production worldwide. The global market places value on longer and stronger fibers, mandating U.S. breeders develop cultivars to meet this demand and remain competitive with man-made fibers.

One challenge breeders face concerning the improvement of fiber quality traits is low genetic diversity among elite, agronomically acceptable genotypes of upland cotton. The use of DNA marker-assisted selection could help breeders access unexploited genetic diversity as well as facilitate the simultaneous improvement of both yield and fiber quality traits, he said.

Recent quantitative trait loci, or QTL, mapping studies have led to the discovery of hundreds of QTL for fiber length and strength, and many of them show promise for use in marker-assisted selection, Smith said.

However, there are few reports of public programs utilizing marker-assisted selection for the improvement of fiber quality traits, he said. In general, there has been inconsistency among QTL discovered across studies and the QTL discovered generally are considered specific to the mapping populations in which they were discovered. Both are major obstacles preventing the use of marker-assisted selection for fiber quality.

Smith, along with Hugie, a Cotton Incorporated Fellow and doctoral student, began the project, “Identification of Robust Microsatellite Markers for Fiber Quality in Gossypium hirsutum,” to determine which of the publically available markers can be used in marker-assisted improvement of fiber quality in his program.

“The U.S. exports most of its cotton, because companies find it more economical to export our fiber, process it in other countries, and then import the finished textiles back into the U.S.,” Hugie said. “Thus, the U.S. now competes with raw cotton production globally, and improved fiber length will help our producers compete in this global marketplace.”

Hugie said in her research, she was looking for segments of DNA on cotton chromosomes related to fiber length and strength.

“We were looking for landmarks, or DNA markers, on chromosomes that we could use to identify these traits,” she said. “Instead of harvesting the cotton and sending fibers to a lab for fiber quality analysis to get information on length and strength, maybe we could just obtain the DNA and harvest only desirable plants without having to harvest the undesirable plants and thus save labor cost and improve breeding efficiency.

“What we found were DNA markers that are stably associated with fiber quality traits across diverse genetic backgrounds in the breeding program, but individually they really don’t increase length or strength tremendously,” Hugie said.

“But, research continues at Texas A&M with Dr. Hongbin Zhang and others to identify multiple QTL that together could provide selection techniques giving major improvements,” Smith said.

Zhang is professor of plant genomics and systems biology and director of the Laboratory for Plant Genomics and Molecular Genetics in College Station.

The study screened a total of 223 publically available DNA markers. Of that number, 55 markers were significantly associated with fiber length and 50 markers were significantly associated with fiber strength. Those numbers were narrowed down to subsets of three or four markers, which were then used in the selection of progeny rows across three different populations in the breeding program, Hugie said.

There was a significant difference in the mean fiber length and fiber strength between marker selected and non-selected progeny rows, she said. They also observed a differential response to marker-assisted selection across the three populations, which most likely is attributable to genetic background effects.

Still, Hugie said, the results demonstrate that the identified markers for fiber length and strength are reasonably stable across diverse genetic backgrounds.

“Our results suggest that the current, publicly available markers for fiber quality QTL are not yet a cost-effective alternative to field-based selection for the improvement of fiber quality considering the minor increase in trait means observed and the small amount of variation in length and strength accounted for by the markers,” Smith said.

“As genotyping costs continue to decrease relative to phenotyping costs, molecular breeding approaches that better capture phenotypic variation across different genetic backgrounds, such as mapping-as-you-go and genomic selection, may prove to be more efficient and cost effective methods for the improvement of fiber quality in cotton,” he said.

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Farm & Ranch

Grazing North Texas: Who’s Your Neighbor?

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By Tony Dean, tonydean.tx1@gmail.com

Who qualifies as your neighbor? Webster says it is someone who lives nearby, but it isn’t always that simple. Is my neighbor the person next door, or someone in the next county? It could be both.

Our neighbors are an important part of life on the ranch and the grazing lands we share. Many of us find comfort in knowing another human being is somewhere in our neck of the woods, and that someone nearby cares what happens to us.

Being a good neighbor is part of being a productive and responsible rancher, so let’s look at some of the qualities that make a good one.

Protect your neighbor

Watch out for him.

The cattle business is dangerous. Almost everything we work with is bigger and faster than we are. A mad cow can turn an otherwise wonderful day into a disaster. Sooner or later, all of us will need some help.

The average age of today’s rancher keeps climbing. Be aware of your neighbors’ ages, limitations or disabilities, and keep an eye out for anything outside their normal routine.

More women own and operate ranches today, too. Some may suddenly find themselves responsible for parts of the operation that a spouse or family member used to handle. As neighbors, we can be a valuable backup system, helping one another learn, adjust and keep the operation moving.

And then there is wildfire. It can be on us in minutes. You may need to help move a family down the road to safety or move livestock out of harm’s way. Have a plan in mind before the emergency happens.

Be a friend

Some of us who live in the country drive to town for work or make regular trips for other reasons. Others are stay-at-home ranchers, and life can get a little lonely. A friendly smile, a phone call or an invitation to lunch can mean more than we realize.

Things aren’t always wine and roses at the ranch. Few occupations ask people to juggle such a mix of complex markets, weather, natural resource management, livestock management and countless other issues.

Spirits can get low. If we are listening, we can hear and feel the stress in someone’s life. On those days, you have a chance to be a special kind of friend.

Help your neighbor

Have you ever tried to load a trailer by yourself and needed someone to stand in the gate for a couple of minutes? All of us will need help at some point, and our neighbors should be among the people we can call.

Do your best to be there when needed, and your neighbors will likely be there when it is your turn to ask. Sometimes it will be a small job. Other times, it will be a big one. It doesn’t matter. We help our neighbors.

It’s also smart to make sure someone nearby knows your ranching program and understands the land you use. At some point, one of you may need to take care of business for the other for several days.

Be cooperative

There is no end to the situations that can involve more than one family. Fencing, road maintenance and livestock getting where they don’t belong are common ones.

If you don’t handle these things with a friendly, cooperative attitude, they can create problems between neighbors that last for years.

Good fences are something to be proud of. Nothing is gained by years of bickering. Reaching an agreement may be hard at times, but neighbors should be able to work things out. Clear your head, use common sense and come up with an answer everyone can live with.

If your cattle get mixed up with your neighbor’s, make it a priority to separate them as soon as possible. It is rude to let your cattle stay in your neighbor’s pasture eating his grass while you do nothing about it.

One good way to handle strays is for the rancher whose pasture they are in to pen his own cattle along with the strays. The other rancher can then load his cattle and take them home. Afterward, both ranchers can work together on any fence repairs.

By the way, a gate between neighbors is almost always a good policy.

Take ownership

The only way to avoid mistakes is to do nothing. But it isn’t in us as ranchers to sit around and watch the world go by. We are men and women of action, so things are going to go sideways from time to time.

When that happens, and it will, own your mistakes. Decisive action can prevent misunderstandings and make a problem go away much faster.

Be honest, and never, ever lie.

Be patient

We all have bad days. When your neighbor is a little grouchy, he or she probably has something weighing on them.

That is a chance to be a good neighbor. Be patient. Be a good listener. Help your neighbor through the turmoil if you can.

Respect property boundaries

Trespassing is wrong, illegal and rude. Never do it except in an emergency.

In the Southwest, where we live, land ownership is considered a sacred right, and it is a right available to anyone. With ownership comes an expectation of privacy and respect. When you trespass, you violate those rights.

And yes, it is trespassing when you cross a fence to fish or hunt without permission. Texas is a private-property state, which makes respecting these boundaries especially important.

That doesn’t mean you can’t have an understanding with your neighbors that lets you onto their land. Many good friends and neighbors work together on one another’s ranches, but both parties should always communicate well.

You have a cell phone. Use it any time you doubt whether you have the right to go through the gate. An invitation onto another person’s ranch is a privilege, so don’t abuse it.

Your word

Your word as a rancher, neighbor, man or woman helps define you in your community and throughout your life. Your neighbors want to trust you. They want to know that all they need from you is a handshake and that they can depend on what you say.

It is a blessing to live in North Texas, where people still respect and honor a life lived with truth and love.

A good life to live

Having good neighbors is a great way to live. It brings comfort and enjoyment to life. Sometimes it takes a little extra effort, but a friendly neighborhood, even one that covers many acres, is enjoyable and can help keep all of us safer.

It is worth the effort.

The Bible

The Bible has many references to being a good neighbor. Being a good neighbor was good judgment 2,000 years ago, and it is good judgment today. Jesus made it clear that love should guide a good neighbor.

Leviticus 19:18 tells us to love our neighbors as ourselves. That is a tall order. In Matthew 5:44, Jesus takes it further, telling us to love our enemies and pray for those who persecute us.

Our goal should always be to love one another, lift each other up, and help and protect our neighbors.

All of us face drought, floods, wildfire, tornadoes, low cattle prices, rattlesnakes and feral hogs, so let’s fight the fight together. Stand by your neighbor’s side, and he will stand by yours.

A North Texas minister was recently asked, “What do you do if you have a neighbor who is really hard to love?”

His answer was short and to the point: “Love him anyway.”

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Farm & Ranch

Beyond Crude Protein

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By NTFR Staff

A stack of hay may look good from the outside. The bales may be heavy, green and free of obvious weeds or mold. None of that tells a cattle producer what the hay will provide once it reaches the feed bunk.

Two bales from the same field can differ noticeably in nutrition if they were cut at different stages of maturity, weathered differently or stored under different conditions. The differences grow even larger when you compare hay bought from separate farms or produced in different growing seasons.

A forage analysis takes much of the guesswork out by showing what the hay contains and where you may need to supplement.

Crude protein is often the first number producers check, but it is only one part of the report. Energy, fiber, moisture and possible toxicity concerns can matter just as much when deciding whether a lot of hay suits dry cows, lactating cows, replacement heifers or growing calves.

Reading beyond the protein number

Most forage reports show results on both an “as received” and a dry-matter basis. The as-received column includes the water in the hay. The dry-matter column takes moisture out of the calculation so you can compare feeds with different moisture levels fairly.

For ration planning, the dry-matter values are usually the most useful. If you’re comparing dry hay with silage, cubes or another feed, make the comparison on a dry-matter basis rather than on the numbers as fed.

Moisture still deserves attention. Hay baled too wet is more likely to heat, mold or deteriorate in storage. In severe cases, heating can create a fire risk. Moisture also affects how many pounds of actual nutrients you are buying. A heavy bale isn’t necessarily a nutrient-dense bale if much of its weight is water.

Crude protein, usually listed as CP, estimates the protein in the forage based on its nitrogen content. Protein keeps the rumen microbes that help cattle digest forage working. When forage protein drops too low, intake and digestion may decline because those microbes can’t function as well.

The amount of protein a cow needs changes a great deal through the year. A mature, dry cow in mid-pregnancy needs less than a cow in late pregnancy or early lactation. Young, growing cattle and first-calf heifers may also need more protein than mature dry cows. Hay that is adequate for one group may fall short for another.

Always compare a forage report with the needs of the cattle that will eat the hay. Oklahoma State University notes that cattle requirements vary with weight, age, stage of production, desired gain and milk production. A single protein target can’t describe the needs of an entire herd.

Energy is another major piece of the feeding decision. Many forage reports express energy as total digestible nutrients, or TDN, an estimate of the digestible energy in the feed. Protein supports rumen function and tissue growth, while energy fuels maintenance, pregnancy, milk production and weight gain.

Hay can test well for protein and still come up short on energy. That matters most for cows nearing calving or producing milk. Low-energy hay may leave cattle feeling full without giving them enough nutrition to hold body condition. University of Nebraska Extension stresses evaluating both protein and energy when judging the feeding value of forage.

Fiber measurements help explain why cattle eat one hay readily and less of another. Neutral detergent fiber, or NDF, represents much of the plant’s structural material, including cellulose, hemicellulose and lignin. As NDF rises, cattle generally eat less because bulky, fibrous forage stays in the digestive tract longer.

Acid detergent fiber, or ADF, includes the least digestible parts of the plant. Higher ADF generally means lower digestibility and less available energy. Put simply, high NDF can limit how much cattle eat, and high ADF can mean they get less energy from what they do eat.

Both numbers tend to climb as forage plants mature. Mature hay usually has more stem and less leaf than hay cut earlier. It may still be acceptable feed for animals with lower needs, but it is less likely to support lactating cows or growing cattle without supplementation.

Some reports also include relative feed value, or RFV. This index uses ADF and NDF to compare forage quality, particularly for alfalfa and other legumes. It can help when you compare similar types of hay, but it doesn’t account for every nutrient cattle need. Don’t let RFV replace a closer look at crude protein, energy and the animal’s stage of production.

Depending on the lab package you choose, the report may include minerals. Labs commonly report calcium and phosphorus, but a forage analysis can’t replace a well-designed mineral program. Water quality, other feeds and interactions among minerals all affect mineral needs and availability. Work with an Extension specialist, veterinarian or livestock nutritionist before making major mineral changes.

Nitrate and prussic acid

A standard forage analysis may not include nitrates, and you often have to request that test separately. Nitrate testing is especially important for drought-stressed forage, heavily fertilized fields and crops such as sorghum, sudangrass, sorghum-sudan hybrids, millet, oats and some weeds.

Nitrate can remain in cured hay and doesn’t reliably disappear in storage. Test any hay you suspect of high nitrate before feeding it. Oklahoma State University recommends a laboratory analysis because field screening tests can show that nitrate is present but can’t measure how much.

Prussic acid is a different concern. Unlike nitrate, prussic acid usually dissipates as properly cured hay sits in storage, though suspect forage still needs careful management and professional guidance. Be sure which test you need, and don’t treat the terms nitrate and prussic acid as interchangeable.

Putting the numbers to work

A hay test pays off when it leads to a feeding plan. Once you have the results, you can match the hay to the right class of cattle. Save better hay for replacement heifers, thin cows, late-gestation cows or cows with calves at side. Feed lower-quality hay to mature dry cows with modest requirements.

Testing can also prevent unnecessary supplementation. Without an analysis, you may buy protein tubs, cubes or other supplements your cattle don’t need. The opposite mistake costs just as much. Assuming hay is better than it is can lead to lost body condition, lower milk production, poor breed-back rates and lighter calves.

Visual inspection still matters. Don’t ignore mold, foreign material, weeds, dust or heat damage because a lab report shows acceptable numbers. At the same time, color and appearance can’t reliably predict nutrient content. University of Nebraska notes that forage nutrient values vary widely, which makes average book values a poor substitute for testing the feed you have on hand.

A forage analysis won’t make poor hay better, but it can show you how to use that hay well. For the cost of a test, you get information that can guide hay purchases, cut supplement waste and put each lot of hay where it will do the most good.

The protein number may get the most attention, but it doesn’t tell the whole story. Understanding moisture, TDN, NDF, ADF and potential toxicity gives you a much clearer picture of what is inside the bale.

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Farm & Ranch

Don’t Be Caught Off Guard

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By Savannah Magoteaux

A September rain can be exactly what a North Texas pasture needs, but that flush of green can bring an unwelcome guest.

Fall armyworms are a familiar late-summer and early-fall pest across Texas, and they can do serious damage to pastures and hay fields when conditions are right. Bermudagrass is a common target, but armyworms also feed on small grains, ryegrass and other forage crops. The key is catching them early, before a manageable infestation turns into a field that seems to disappear overnight.

Despite the name, fall armyworms are caterpillars. The adult moths migrate into Texas and lay masses of eggs on grasses, fence posts, buildings and other surfaces. Once the larvae hatch, they start feeding on plants.

Young armyworms are small and hard to spot unless you are actively scouting. As they grow, their appetite climbs. Larger larvae can strip leaves fast, leaving stems behind and cutting into available forage or hay yield.

Look for an inverted, light-colored “Y” on the caterpillar’s head. Mature larvae can reach about 1½ inches long and often have several dark and light stripes running the length of the body.

Armyworms feed most actively during the cooler parts of the day, so early morning and late afternoon are good times to scout. Walk through several areas of the pasture rather than checking only along the edge. Look closely near the soil surface and beneath grass leaves, especially where you see fresh feeding damage.

Scouting matters most after rain or irrigation. New grass growth draws newly hatched larvae, and populations can build quickly when conditions favor them.

Texas A&M AgriLife Extension generally recommends considering treatment in improved pastures when you find about three or more armyworms per square foot. The decision should also weigh the size of the larvae, how much forage is present, the expected hay value and how severe the damage is.

Timing matters. Small armyworms are easier to control than large ones, and an insecticide does less good once the caterpillars are nearly mature and have already eaten much of the forage. If you treat, follow the product label carefully, including grazing and hay-harvest restrictions.

Don’t assume one treatment or one scouting trip will carry a pasture through the season. Fall armyworm moths can keep moving into an area, laying more eggs and starting another generation of larvae. Keep checking fields even after you have controlled an infestation.

For hay producers, an approaching harvest date can also shape the decision. When the crop is close to harvest, cutting the field may be a better option than spraying. The best choice depends on the number and size of the armyworms, how much forage remains and whether the weather allows you to cut hay.

September rain is usually welcome across North Texas, especially after a long, hot summer. Just remember that fresh green grass deserves a closer look. A few minutes of scouting can make the difference between catching fall armyworms early and finding the damage after they have eaten their fill.

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